ABSTRACT
This collection of research articles and reviews covers the latest work in the design, delivery, dynamic abilities, and immune stimulation of RNA nanoparticles which have driven the utilization of their immunomodulatory properties. The unknown immune properties of nucleic acid nanoparticles have been a major hurdle in their adaptation until the works herein began assessing their structure-activity relationships. This collection chronologically follows the path of investigating the recognition of design components to implementing them into nucleic acid nanostructures.
RNA nanotechnology is an emerging platform for therapeutics with increasing clinical relevance as this approach becomes more widely used and approved for the treatment of various diseases. The latest research aims to take advantage of RNA’s modular nature for the design of nanostructures which can interact with their environments to communicate programmed messages with intracellular pathways. In doing so, nanoparticles can be used to elicit or elude responses by the immune system as desired in conjunction with their therapeutic applications.
TABLE OF CONTENTS
part I|246 pages
Nanodesign
chapter Chapter 5|26 pages
Multistrand RNA Secondary Structure Prediction and Nanostructure Design Including Pseudoknots*
chapter Chapter 6|29 pages
Computational and Experimental Characterization of RNA Cubic Nanoscaffolds*
chapter Chapter 8|23 pages
Ring Catalog: A Resource for Designing Self-Assembling RNA Nanostructures*
chapter Chapter 10|24 pages
Versatile RNA Tetra-U Helix Linking Motif as a Toolkit for Nucleic Acid Nanotechnology*
part II|377 pages
Functional RNA Nanoparticles and Their Delivery
chapter Chapter 11|36 pages
Design and Self-Assembly of siRNA-Functionalized RNA Nanoparticles for Use in Automated Nanomedicine*
chapter Chapter 12|10 pages
Co-transcriptional Assembly of Chemically Modified RNA Nanoparticles Functionalized with siRNAs*
chapter Chapter 15|31 pages
Bolaamphiphiles as Carriers for siRNA Delivery: From Chemical Syntheses to Practical Applications*
chapter Chapter 20|26 pages
Exosome Mediated Delivery of Functional Nucleic Acid Nanoparticles (NANPs)*
part III|258 pages
Dynamic Nucleic Acid Nanoparticles
chapter Chapter 23|24 pages
Activation of Different Split Functionalities on Re-association of RNA–DNA Hybrids*
chapter Chapter 24|29 pages
Co-transcriptional Production of RNA–DNA Hybrids for Simultaneous Release of Multiple Split Functionalities*
chapter Chapter 25|25 pages
Triggering of RNA Interference with RNA–RNA, RNA–DNA, and DNA–RNA Nanoparticles*
chapter Chapter 26|28 pages
Multistrand Structure Prediction of Nucleic Acid Assemblies and Design of RNA Switches*
chapter Chapter 27|20 pages
The Use of Minimal RNA Toeholds to Trigger the Activation of Multiple Functionalities*
chapter Chapter 28|24 pages
Functionally-Interdependent Shape-Switching Nanoparticles with Controllable Properties*
chapter Chapter 29|25 pages
Dynamic Behavior of RNA Nanoparticles Analyzed by AFM on a Mica/Air Interface*
chapter Chapter 30|19 pages
Broccoli Fluorets: Split Aptamers as a User-Friendly Fluorescent Toolkit for Dynamic RNA Nanotechnology*
chapter Chapter 31|58 pages
Aptamers as Modular Components of Therapeutic Nucleic Acid Nanotechnology*
part IV|288 pages
Immunomodulation with NANPs